Nikolaus Grigorieff, PhD

Nikolaus Grigorieff wants to visualize cellular macromolecular assemblies in atomic detail, to better understand the mechanisms underlying their function. To do this, Grigorieff and his team develop methods in cryo-electron microscopy (cryo-EM) that use a “single-particle” approach, eliminating the need for crystallization. Recently, the team and collaborators have begun developing a method to detect molecules and assemblies in situ – inside cells – for a more complete spatial understanding of local distributions and to preserve weak and transient interactions otherwise missed. Grigorieff makes the tools developed in his lab available to other scientists, including open-source software to process cryo-EM data.