Human cells consume a wide variety of nutrients and transform them into new molecules, serving specialized functions in bioenergetics, biosynthesis, and signaling. The fate and function of these nutrients change profoundly in human diseases. Interestingly, there are hundreds of thousands of these small molecules in our bodies, yet the identities of many of them remain unknown. To address this gap in knowledge, the Spinelli lab leverages an innovative platform using high-resolution mass spectrometry to discover and characterize previously unknown human metabolites. Identifying these molecules will help advance basic understanding of human biology. Further, it could also unveil mechanisms of disease pathogenesis and point to novel therapeutic strategies.