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Eric Betzig received the 2014 Nobel Prize in chemistry for developing super-resolution fluorescence microscopy. At UC Berkeley, Betzig helps lead new initiatives in imaging technology, particularly at the intersection of physics, biology, and terabyte-scale image analysis. Research in the Betzig Lab at Janelia is advancing the understanding and application of multiple imaging technologies including: lattice light sheet, adaptive optics, cryogenic correlative light and electron microscopy, and live cell structured illumination microscopy. These advances are allowing scientists to reveal and decipher the structure and dynamics of living cells and tissues in unprecedented detail.