Elizabeth Villa wants to see inside cells at the highest possible resolution to understand the structural dynamics and interactions of macromolecular complexes. To accomplish this, Villa and her team develop tools and technologies to bridge live-cell imaging with cryo-electron tomography (cryo-ET). Then, they use these tools to explore fundamental or previously unexplored questions in cell biology. For example, the team is focused on understanding complex biology in prokaryotes and eukaryotes, such as studying a kinase and its cellular function in health and its role in Parkinson’s disease. In addition, they are examining the molecular architecture of the nuclear periphery and are investigating dynamic processes in bacteria.
A team of researchers led by HHMI Investigator Michael Rosen has captured the most detailed images yet of the molecules in chromatin condensates — droplet-like structures of compacted DNA. Thirteen project teams involving 70 scientists from 29 institutions are collaborating on basic research targeted at preparedness for future emerging pathogens that could threaten human health. The large viruses known as jumbo phages employ a curious counter-defense strategy to protect their DNA while attacking bacteria. Now, scientists have identified the key protein involved and solved its structure.