Not two Shakespearean witches from Macbeth, but a side (left image) and a bottom view (right image) of the same midge pupa imaged with a high resolution microscope. These haunting views show that inside the pupa of the midge, the adult body, including the compound eyes, the cloak-like wings, and the legs, are fully formed and ready to emerge. Midges spend the winter as larvae in the water, pupate, and emerge from the water in the spring. The adult life cycle takes only about 2 to 3 weeks in the summer, often in large swarms that facilitate mating.
Photo courtesy of Igor Siwanowicz PhD, Janelia Farm Research Campus, Ashburn, VA
The pupa was fixed and stained with dyes that highlight the cuticle and muscle. The image was collected with a laser scanning confocal microscope (magnification around 100x).