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Lynne Chantranupong knows how to get cells to spill their secrets. She has characterized key regulators of a signaling pathway that tells cells to grow, a process that goes awry in cancer and diabetes. Now, she is setting her sights on the brain. Chantranupong plans to isolate intracellular packets that contain neurotransmitters, signaling molecules that carry messages between nerve cells. She wants to probe the contents of these packets using mass spectrometry. This high-resolution method promises to reveal a complex and dynamic atlas of neurotransmitters in the brain.