Molecular Biology, Neuroscience
The Rockefeller University
Dr. Vosshall is also Robin Chemers Neustein Professor and head of the Laboratory of Neurogenetics and Behavior at the Rockefeller University.
Leslie Vosshall is studying the molecular and neural mechanisms underlying chemosensory perception in fruit flies, mosquitoes, and humans. For these animals, environmental odors are crucial as they make appropriate decisions about food choice and mating partners. Understanding how mosquitoes select human hosts will have important implications for infectious diseases spread by blood-feeding insects.
For some people, the steroid androstenone, which is a component of male sweat, has a sweet or even pleasant floral scent. Other people can't smell it at all. But when Leslie Vosshall sniffs it, she recoils in disgust, likening it to the smell of a sweaty armpit.
Vosshall has discovered that the different olfactory responses to this chemical are strongly influenced by a person's genetic makeup. In 2007, her lab and that of her Duke University collaborator Hiroaki Matsunami published a report that revealed that people who hate the smell are more likely to carry two good copies of a gene for OR7D4, an olfactory receptor in the nose. Individuals who do not find the smell of androstenone offensive tend to lack one or both good copies of the gene.
Vosshall, who is on the faculty at the Rockefeller University, believes this research may describe the first-known genetic variation underlying individual differences in odor perception. The idea for the experiment was born of the same probing curiosity that has fueled Vosshall's research on other aspects of smell—the fascinating but least understood of the senses.
The majority of her research involves not humans, however, but insects like the fruit fly and mosquito. They serve as relatively simple models for probing how the brain and nervous system transform olfactory cues into specific behaviors. Moreover, some of the insects she studies are pests that devour crops or spread infectious diseases, which can have devastating economic and health consequences. The discoveries Vosshall is making about how these insects detect odors and how odors influence their behavior may help researchers identify new ways to fend off the insects.
In the 1990s, as a postdoctoral fellow in the lab of Nobel laureate Richard Axel, an HHMI investigator at Columbia University, Vosshall was excited by the series of landmark discoveries that Axel's group was making about the way odors are detected, encoded, and perceived by the olfactory system. Working with Axel, she identified a large family of genes in the fruit fly that function as odor receptors.
As an independent scientist at Rockefeller, she created a nearly complete map of the fruit fly's olfactory system and identified odorant receptors in fruit fly larvae. To the surprise and consternation of many in the field, Vosshall found that insects have evolved a set of smell receptors unlike those found in other animals and humans. Instead of attaching to G protein-coupled receptors, odorant molecules are detected in insects by transmembrane receptors that seem to function as ion channels. (G protein-coupled receptors are a large family of transmembrane receptors that sense molecules outside the cell and activate signaling pathways and other cellular responses inside the cell.)
It has long been known that mosquitoes are attracted to the carbon dioxide that humans exhale, and in 2007, Vosshall identified two membrane proteins in fruit flies and mosquitoes that detect this gas. Still, Vosshall does not believe that carbon dioxide is the only thing that attracts mosquitoes to humans. After all, she says, mosquitoes seem to go after some individuals voraciously while others say they're rarely bitten—yet everyone emits carbon dioxide. "If it were just carbon dioxide, mosquitoes would be falling into your beer," she says.
As an HHMI investigator, Vosshall says she would like to make the mosquito a viable model organism for asking scientific questions. She acknowledges that the proposal is a significant departure from her current research, but she believes the time is right for such a giant step. She sees a tremendous opportunity to apply the advanced transgenic technology currently used routinely in other insect models to aid in understanding the female mosquito's life cycle and what drives it to bite humans. Likewise, she notes, there's a wealth of information in the mosquito genome sequence data that may help researchers develop, among other things, more targeted, less toxic insect repellents.
One point of attack might be the protein Or83b, which Vosshall found is expressed in nearly all olfactory neurons of the fly and acts as a generic coreceptor in tandem with specific odorant receptors. "If you make fruit flies without the Or83b gene they can't smell," she says. "And if you put the gene back into this fly, you cure its smell problem."
That revelation led to Vosshall's publication of a research article in early 2008 that showed for the first time how the commonly used insect repellent DEET works. DEET was discovered by the United States Army in 1957, and although it is highly effective and heavily used worldwide, researchers did not have any idea how it worked. Vosshall's experiments showed that DEET acts on pairs of olfactory receptors that include Or83b.
Thus the Or83b gene/protein is a "cornerstone" of insect olfaction, Vosshall adds, suggesting that blocking Or83b might be a selective and "clean" strategy for repelling harmful insects. "A Medfly, for example, wouldn't be able to smell citrus crops," she says. Or in areas of endemic malaria, mosquito netting might be impregnated with a compound that inhibits this vital olfactory protein, effectively "blinding" mosquitoes to the scent of their human targets.
With so many infectious diseases transmitted by insect vectors, Vosshall is hoping her research may contribute to lessening the burden of illness in the developing world.