Plot of fluorophore brightness (ε × Φ) versus the wavelength of maximum absorption (λ_max) for the major classes of fluorophores. The color of the structure indicates its wavelength of maximum emission (λ_em). For clarity, only the fluorophoric moiety of some molecules is shown.

From Lavis, L.D., and Raines, R.T. 2008. ACS Chemical Biology 3:142–155, © 2008 American Chemical Society.