Joseph Takahashi is interested in understanding the genetic and molecular basis of circadian rhythms as well as other complex behaviors. He uses forward genetic approaches in the mouse to discover genes regulating the nervous system and behavior. The molecular mechanisms of the clock are being studied at the biochemical and genomic levels.
Neurogenetics of Circadian Rhythms and Behavior in Mice
Comparison of circadian activity rhythms and responses to psychostimulants in different inbred strains of mice reveals wide phenotypic variation in both of these behaviors. We combine information on the haplotype structure of the mouse genome with high-resolution SNP (single-nucleotide polymorphism) analysis to identify circadian loci using both linkage and association methods. Students will be involved in genotyping, SNP analysis, genomic sequence analysis, and candidate gene analysis.
Genome-Wide Analysis of Circadian Gene Expression in the Mouse
Circadian control of gene expression is pervasive—approximately 10–20 percent of transcripts expressed in cells and tissues are under circadian regulation. Students will be involved in bioinformatic analysis of genome-wide circadian expression in the mouse. Projects involve RNA profiling using RNA-seq, analysis of RNA processing, global circadian transcription factor analysis, and chromatin remodeling.