John Kuriyan is interested in the structure and mechanism of the enzymes and molecular switches that carry out cellular signal transduction and DNA replication. His laboratory uses x-ray crystallography to determine the three-dimensional structures of proteins involved in signaling and replication, as well as biochemical, biophysical, and computational analyses to elucidate mechanisms.
Structural and Functional Studies of Cellular Signaling Enzymes
The student will be engaged in research aimed at understanding the structural mechanisms by which enzymes, such as tyrosine kinases, promote cellular signal transduction. The student will conduct laboratory procedures, including molecular cloning, protein expression and purification, biochemical activity assays, and crystallization screening. In addition, the student may also participate in crystallization optimization, x-ray diffraction data collection, and molecular model building, depending on the progress of the project.