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Princeton Lab Protocols
This website contains a series of experimental laboratory protocols, developed for high school teachers participating in Princeton University’s summer Molecular Biology Outreach Program, which can be downloaded, adapted, and edited for the classroom. The 2008 experiments, called "Cool Genes," illustrate the power of state-of-the-art molecular biological techniques in the physical mapping of genes, the analysis of mutations, and the determination of inheritance. Five labs are on the site. For example, Laboratory 1 demonstrates the "mega-methods" of molecular biology with an analysis of DNA using restriction mapping and gel electrophoresis. Laboratory 2 deals with isolating metagenomic DNA from contaminated soil. Past protocols, which include cloning and PCR, are also available on the website. The site also contains “classroom ready” protocols, such as a DNA purification protocol for evidence of genetic modification in grocery store foods, in both Word and PDF formats.
Laboratory 1: The Mega-Methods of Mol Bio: Analysis of DNA Using Restriction Mapping and Gel Electro
This laboratory protocol is an example of the general theme of the website: All the labs emphasize a basic understanding of the relationship between DNA structure, modern genetics, and several social and ethical concerns.
Media: WordProgram Director: Frederick Hughson, Ph.D.
Award Years: 1989, 1994, 1998, 2002, 2006
Summary: Princeton University is a private research institution in Princeton, New Jersey. Its HHMI-funded initiatives include:
- An intensive Summer Program which gives students the chance to conduct cutting-edge scientific research and opportunities to make well-informed decisions about a career in the sciences;
- A summer institute for secondary school science teachers that includes seminars by university faculty and cooperative laboratory experiences and learning; and
- A molecular biology laboratory that has provided hands-on laboratory experience to more than 3,500 students and will support a new curriculum that will expose biology students to courses now only taught to engineering and physics students.