Research Specialist - GENIE Project

Job Type:


Employment Category:

Full Time (40 hours/week)


Janelia Farm Research
Ashburn, Virginia

Job Number:


Posted On:


Reports To:

Project Scientist II

Job Summary:

The Research Specialist will produce thousands of DNA constructs for expression of genetically-encoded neuronal activity sensor variants in several biological systems including bacteria, mammalian neurons cultured in vitro, the Drosophila nervous system, the C. elegans nervous system, and the mouse nervous system. This will entail large-scale, site-directed mutagenesis of sensor sequences following both directed and unbiased design strategies in a high throughput cloning effort. Additionally, the Research Specialist will aid in screening genetically-encoded neuronal activity sensor variants in functional in vitro tests including biochemical assays using purified protein preparations.

Principal Responsibilities:

  • Production of thousands of genetically-encoded neuronal activity sensor variant DNA constructs
  • Site-directed mutagenesis of cDNAs
  • Subcloning of genetically-encoded neuronal activity sensor constructs into bacterial, invertebrate, and mammalian expression vectors
  • Perform high volume DNA sequencing and analysis
  • Protein purification
  • Western blotting
  • In vitro assay of genetically-encoded neuronal activity sensor variants in purified protein preparations
  • Set-up, execute, and optimize high throughput cloning protocols
  • Record and communicate data in laboratory notebooks, written reports, and oral presentations
  • Report the work done and the methods used to the Project Scientist
  • Contribute to team meetings and planning

Preferred Qualifications:

Education / Experience:

  • Bachelor’s degree in the life sciences required
  • Master’s degree in the life sciences preferred
  • Expert level molecular biologist with greater than 5 years of laboratory experience
  • Experience scaling up molecular biology tasks using automation and protocol optimization
  • Protein engineering/molecular evolution experience preferred
  • Tissue culture experience preferred
Skills and Abilities:
  • Perform complex motor tasks with speed and precision handling small volumes of reagents
  • Safely and accurately perform laboratory bench work for up to 8 hours per day
  • Independent design and analysis of DNA constructs
  • Utilize sequence analysis software
  • Focus attention on a repetitive task for extended periods
  • Excellent oral and written communication skills
  • Keep accurate and organized records of work performed

Additional Information:

Please upload a cover letter and the names and contact information of three references with your resume. Please apply online at https://janeliacareers.silkroad.com.

To Apply:

To apply for this position, please email your resume to:

Veronica Dunn
Sr. HR Specialist, TA
19700 Helix Drive
Ashburn, Virginia 20147
Click Here to Apply

Application Deadline:

Open Until Filled

HHMI is an Equal Opportunity Employer