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February '06
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Scientists Crack Code for Motor Neuron Wiring

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What's more, it became clear from the pictures that certain pairs of Hox proteins exclude each other from an individual neuron, whereas other combinations of Hox proteins can coexist. In effect, the Hox proteins wage a battle for dominance within the cells of each pool. "Hox protein A may win out in one neuron and Hox protein B may prevail in another neuron," says Jessell. "And since the emergence of that final Hox pattern determines identity, the nature of the interactive circuit between Hox proteins is in itself driving the diversification of neurons."

These findings may have broad implications. "We already know that the basic organization of the chick motor system is conserved within higher vertebrates, including humans," Jessell predicts. "If you look at chick locomotor behavior, it's strikingly similar to humans walking." It's possible, then, that understanding the Hox code may one day help guide progress in restoring motor neuron function in people whose spinal cords have been damaged by trauma or disease. grey bullet

Outfitting Motor Pools
Outfitting Motor Pools

Researchers in Thomas Jessell's lab have created an atlas of images that shows the location of various Hox-related proteins in the chick embryo at different developmental times.

For this set of images, the researchers injected different muscles in the chick forelimb with a green tracer, which then spread to the corresponding motor neuron pools in the spinal cord.

Each panel shows cell nuclei stained with red-tagged antibodies that bind to different transcription factors. The transcription factors, shown clockwise from top left, are Runx1, Pea3, Scip, and Pea3.

The images demonstrate that different combinations of transcription factors are present in different motor neuron pools.

Images: Jeremy Dasen/Jessell Lab

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HHMI INVESTIGATOR

Thomas M. Jessell
Thomas M. Jessell
 
Related Links

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Scientists Crack Code for Motor Neuron Wiring
(11.03.05)

ON THE WEB

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Motor Neuron Diseases Information Page: National Institute of Neurological Disorders and Stroke

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